Discovery and Characterization of the 3-Hydroxyacyl-ACP Dehydratase Component of the Plant Mitochondrial Fatty Acid Synthase System1[OPEN]
نویسندگان
چکیده
Department of Biochemistry, Biophysics, and Molecular Biology (X.G., H.J., B.J.N.), National Science Foundation Engineering Research Center for Biorenewable Chemicals (X.G., B.J.N.), Department of Statistics (A.L., D.N.), Department of Genetics, Development, and Cellular Biology (L.L.), Laurence H. Baker Center for Bioinformatics and Biological Statistics (X.Z.), and Center for Metabolic Biology (B.J.N.), Iowa State University, Ames, Iowa 50011; Metabolomics Research Group, RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Japan (Y.O., K.S.); and Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675, Japan (K.S.)
منابع مشابه
The 3-hydroxyacyl-ACP dehydratase component of the plant mitochondrial fatty acid synthase system
متن کامل
Discovery and Characterization of the 3-Hydroxyacyl-ACP Dehydratase Component of the Plant Mitochondrial Fatty Acid Synthase System.
We report the characterization of the Arabidopsis (Arabidopsis thaliana) 3-hydroxyacyl-acyl carrier protein dehydratase (mtHD) component of the mitochondrial fatty acid synthase (mtFAS) system, encoded by AT5G60335. The mitochondrial localization and catalytic capability of mtHD were demonstrated with a green fluorescent protein transgenesis experiment and by in vivo complementation and in vitr...
متن کاملIdentification of the Leishmania major Proteins LmjF07.0430, LmjF07.0440, and LmjF27.2440 as Components of Fatty Acid Synthase II
Leishmania major causes leishmaniasis and is grouped within the Trypanosomatidae family, which also includes the etiologic agent for African sleeping sickness, Trypanosoma brucei. Previous studies on T. brucei showed that acyl carrier protein (ACP) of mitochondrial fatty acid synthase type 2 (FASII) plays a crucial role in parasite survival. Additionally, 3-oxoacyl-ACP synthase TbKASIII as well...
متن کاملMitochondrial fatty acid synthesis and maintenance of respiratory competent mitochondria in yeast.
Mitochondrial FAS (fatty acid synthesis) of type II is a widely conserved process in eukaryotic organisms, with particular importance for respiratory competence and mitochondrial morphology maintenance in Saccharomyces cerevisiae. The recent characterization of three missing enzymes completes the pathway. Etr1p (enoyl thioester reductase) was identified via purification of the protein followed ...
متن کاملIdentification of a novel mycobacterial 3-hydroxyacyl-thioester dehydratase, HtdZ (Rv0130), by functional complementation in yeast.
We report on the identification of Mycobacterium tuberculosis HtdZ (Rv0130), representing a novel 3-hydroxyacyl-thioester dehydratase. HtdZ was picked up by the functional complementation of Saccharomyces cerevisiae htd2Delta cells lacking the dehydratase of mitochondrial type II fatty acid synthase. Mutant cells expressing HtdZ contained dehydratase activity, recovered their respiratory abilit...
متن کامل